Journal: Bioactive Materials

Article Title: Upconversion nanoparticles regulated drug & gas dual-effective nanoplatform for the targeting cooperated therapy of thrombus and anticoagulation

doi: 10.1016/j.bioactmat.2022.03.013

Figure Lengend Snippet: NIR responsive characteristics of UCATS. A) UCL spectra of UCM, UCA and UCATS. B) UV–vis absorption spectra changes of UCA before and after irradiation with 808 nm NIR light (2 W cm −2 ). C) UV–vis absorption spectra changes of SNAP before and after irradiation by vis (room light) and UV (365 nm) light for different time. D) NIR-triggered controllable release of UK from UCATS-UK. E) NIR-triggered NO release from UCATS at different concentrations and SNAP. 808 nm NIR: 2 W cm −2 . F) On/Off behavior of NO release triggered by 808 nm NIR light (2 W cm −2 ). G) The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation (1 W cm −2 ). Scale bar = 50 μm. Data are means ± s.d. (n = 3).

Article Snippet: Ammonium fluoride (NH 4 F, 98.0%) and tetraethyl orthosilicate (TEOS, 99.0%) were purchased from Alfa Co., LTD. DAF-FM DA and Hoechst 33342 were purchased from MCE Co., LTD. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97.0%) was purchased from J&K Scientific.

Techniques: Irradiation, Staining

Journal: iScience

Article Title: Alzheimer's Aβ assembly binds sodium pump and blocks endothelial NOS activity via ROS-PKC pathway in brain vascular endothelial cells

doi: 10.1016/j.isci.2021.102936

Figure Lengend Snippet:

Article Snippet: The cells were washed twice with HBSS containing Mg + and Ca 2+ (HBSS, 09735-75, Nacalai tesque) and were loaded with a fluorescent NO indicator DAF-FM diacetate (SKM423741, Sekisui Medical, Tokyo, Japan), according to the manufacturer’s protocol.

Techniques: Recombinant, Avidin-Biotin Assay, Synthesized, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Derivative Assay, Software, Light Microscopy, Laser-Scanning Microscopy, Imaging, Cytometry

Journal: iScience

Article Title: Alzheimer's Aβ assembly binds sodium pump and blocks endothelial NOS activity via ROS-PKC pathway in brain vascular endothelial cells

doi: 10.1016/j.isci.2021.102936

Figure Lengend Snippet:

Article Snippet: DAF-FM diacetate , Sekisui Medical , Cat# SKM423741.

Techniques: Recombinant, Avidin-Biotin Assay, Synthesized, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Derivative Assay, Software, Light Microscopy, Laser-Scanning Microscopy, Imaging, Cytometry

Journal: Plant Physiology

Article Title: Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots ^{1 [C] [W]}

doi: 10.1104/pp.113.215194

Figure Lengend Snippet: NO production and redox status in Arabidopsis seedlings exposed to cadmium. A, Seven-day-old Arabidopsis wild-type seedlings treated with 0.1 mm cadmium for 24 and 72 h and stained with DAF-FM diacetate for NO quantification. The relative fluorescence values are related to the fluorescence level in control roots (set to 1). False-colored pictures are representative images of transition and elongation root tip zones considered for the data analyses reported in the graph of A. B, Normalized ratio values (405/488 nm) of cytosolic roGFP2 probe in 7-d-old Arabidopsis roGFP2 transgenic seedlings treated with 0.1 mm cadmium for 24 and 72 h. C, Normalized ratio values (405/488 nm) of cytosolic roGFP2 probe in 7-d-old Arabidopsis roGFP2 transgenic seedlings treated with 1 mm 3-AT for 24 and 72 h. False-colored pictures are representative ratiometric images (405/488-nm ratios) of transition and elongation root tip zones considered for the data analyses reported in the graphs of B and C. Asterisks indicate fluorescence levels or normalized ratios that are significantly different from those found in control roots as calculated by Student’s t test (***P < 0.005 and **P < 0.01).

Article Snippet: Detection of NO Production For detection of NO production, 7-d-old Arabidopsis seedlings were incubated with the cell-permeable fluorescent probe DAF-FM diacetate (Alexis Biochemicals), with a final concentration of 5 μ m in the loading buffer solution (0.25 m m KCl, 1 m m CaCl 2 , and 5 m m MES-KOH, pH 5.7) for 15 min. Seedlings were then washed three times with fresh buffer and examined by confocal microscope as previously reported ( Zottini et al., 2007 ).

Techniques: Staining, Fluorescence, Transgenic Assay

Journal: bioRxiv

Article Title: The Effects of Nitric Oxide on Choroidal Gene Expression

doi: 10.1101/2023.06.16.545343

Figure Lengend Snippet: Imaging of intracellular NO with DAF-2DA in chick choroid whole mounts from chick eyes following 3 hrs of recovery from induced myopia and from contralateral control eyes. DAF-2DA labelled cells (green) are readily identified in recovering choroids, but are largely absent in contralateral control choroids. Nucleii are labelled with DAPI. Bar = 40 μM for all images.

Article Snippet: For DAF-FM diacetate (DAF-2DA) labeling, choroids with sclera still attached were placed into a 48-well flat bottom plate (Corning Inc., Corning, NY).

Techniques: Imaging, Control